Human/Mouse MSK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2310
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 261034
Product Specifications
Immunogen
E. coli-derived recombinant human MSK2
Met1-Ser772
Accession # O75676
Met1-Ser772
Accession # O75676
Specificity
Detects human and mouse MSK2 in Western blots. Does not detect recombinant human MSK1.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse MSK2 Antibody
Detection of Human/Mouse MSK2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse MSK2 Monoclonal Antibody (Catalog # MAB2310) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK2 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse MSK2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line
Sample: HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSK2
Mitogen- and stress-activated protein kinase 2 (MSK2), also known as ribosomal protein S6 kinase B (RSKB) and RPS6KA4, belongs to the family of AGC kinases. Both MSK2 and the related MSK1 have two kinase domains connected by a regulatory linker region and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.
Long Name
Mitogen- and Stress-activated Protein Kinase 2
Alternate Names
RPS6KA4, RSKB
Gene Symbol
RPS6KA4
UniProt
Additional MSK2 Products
Product Documents for Human/Mouse MSK2 Antibody
Product Specific Notices for Human/Mouse MSK2 Antibody
For research use only
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