Human/Mouse MSX1 Antibody

Detection of Human MSX1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MSX1 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MSX1 in Human Ovarian Cancer Tissue.

MSX1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) at 0.3, 1.0 and 3.0 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human MSX1 by Western Blot

Regulation of MSX1 expression is mediated by pSMAD1/5/8 in the human cap-stage tooth germs. (A–C) Immunostaining shows that MSX1 expression is abundant in dimethyl sulfoxide (DMSO)-treated human cap-stage tooth germs, dramatically reduced in dorsomorphin treated human cap-stage tooth germ, and unaltered in U0126 + SB203580 + SP600125 treated human cap-stage tooth germs. (D) A Western blot assay confirms the dramatically reduced expression of MSX1 in dorsomorphin treated human cap-stage tooth germs. Actin was used as the internal control. (E) Quantitative analysis of the Western blot assay. de, the dental epithelium; dm, the dental mesenchyme. Error bars represent SD. ***p < 0.001. Bar = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35211032), licensed under a CC-BY license. Not internally tested by R&D Systems.