Human/Mouse NRARP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4814
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NRARP
Ser2-Arg114
Accession # Q7Z6K4
Ser2-Arg114
Accession # Q7Z6K4
Specificity
Detects human and mouse NRARP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse NRARP Antibody
Detection of Human/Mouse NRARP by Western Blot.
Western blot shows lysates of human placenta, liver, and kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NRARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4814) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NRARP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human/Mouse NRARP Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human placenta, liver and kidney tissue
Sample: Human placenta, liver and kidney tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NRARP
NRARP (Notch-regulated ankyrin repeat protein) is a 13 kDa (predicted) protein that is induced by Notch activation. In SDS-PAGE, this protein migrates at 25‑30 kDa. It is expressed in multiple cell types such as fibroblasts and thymocyte precursors. NRARP is presumed to block Notch1-mediated conversion of the CSL transcription factor from a transcriptional suppressor to activator. It is also a positive regulator of the Wnt pathway by stabilizing LEF1. Human NRARP is 114 amino acids (aa) in length. It contains two C-terminal ankyrin-like repeats (aa 50‑82 and 83‑114) that may be involved in protein-protein interactions. Human and mouse NRARP are identical in amino acid sequence.
Long Name
Notch-Regulated Ankyrin Repeat Protein
Alternate Names
MGC61598, NOTCH-regulated ankyrin repeat protein, notch-regulated ankyrin repeat-containing protein
Gene Symbol
NRARP
UniProt
Additional NRARP Products
Product Documents for Human/Mouse NRARP Antibody
Product Specific Notices for Human/Mouse NRARP Antibody
For research use only
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