Human/Mouse PBR Antibody

Name: Human/Mouse PBR Antibody
Brand: R&D Systems
SKU: MAB10045
Rating: 5 (2 reviews)

R&D Systems, part of Bio-Techne | Catalog # MAB10045

(2)

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2393B

View all PBR Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human PBR
Accession # P30536

Specificity

Detects human PBR in direct ELISAs. Detects human and mouse PBR in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse PBR Antibody

Detection of Human and Mouse PBR by Western Blot.

Western blot shows lysates of SK-OV-3 human ovarian adenocarcinoma cell line, MEF mouse embryonic feeder cells, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse PBR Monoclonal Antibody (Catalog # MAB10045) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PBR at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PBR in HeLa Human Cell Line.

PBR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse PBR Monoclonal Antibody (Catalog # MAB10045) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

PBR in Human Prostate Cancer Tissue.

PBR was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human PBR Monoclonal Antibody (Catalog # MAB10045) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm (mitochondria) in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse PBR Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

1 µg/mL
Sample: SK‑OV‑3 human ovarian adenocarcinoma cell line, MEF mouse embryonic feeder cells, and RAW 264.7 mouse monocyte/macrophage cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB10045 in the following applications:

Immunofluorescence (1 Review)
Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PBR

PBR promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism. PBR is expressed at the highest levels under normal conditions in tissues that synthesize steroids. Aberrant expression of PBR has been linked to multiple diseases, including cancer, brain injury, neurodegeneration and ischemia-reperfusion injury.

Long Name

Peripheral-type Benzodiazepine Receptor

Alternate Names

TSPO

Entrez Gene IDs

706 (Human)

Gene Symbol