Human/Mouse Phospho-MSK2/RSKB (S196) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF189
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human and mouse MSK2/RSKB S196 site
Specificity
Detects human and mouse MSK2/RSKB when phosphorylated at S196.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse Phospho-MSK2/RSKB (S196) Antibody
MSK2/RSKB in Human Pancreas.
MSK2/RSKB was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Phospho-MSK2/RSKB (S196) by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human HGF (Catalog # 294-HG) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse Phospho-MSK2/RSKB (S196) Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat dorsal root ganglia and immersion fixed paraffin-embedded sections of human pancreas
Sample: Perfusion fixed frozen sections of rat dorsal root ganglia and immersion fixed paraffin-embedded sections of human pancreas
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human HGF (Catalog #294-HG)
Sample: HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human HGF (Catalog #294-HG)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: MSK2
Long Name
Mitogen- and Stress-activated Protein Kinase 2
Alternate Names
RPS6KA4, RSKB
Gene Symbol
RPS6KA4
Additional MSK2 Products
Product Documents for Human/Mouse Phospho-MSK2/RSKB (S196) Antibody
Product Specific Notices for Human/Mouse Phospho-MSK2/RSKB (S196) Antibody
For research use only
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