Human/Mouse PLC-delta3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5315
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PLC‑ delta3
Lys148-Ala344
Accession # Q8N3E9
Lys148-Ala344
Accession # Q8N3E9
Specificity
Detects human and mouse PLC‑ delta3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse PLC-delta3 Antibody
Detection of Human and Mouse PLC‑ delta3 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse PLC-d3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PLC-d3 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse PLC-delta3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HT‑29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-delta 3
PLC-delta 3 (phospholipase C-delta 3) is an 105 kDa member of the delta-type PLC isozyme family. It is expressed in corneal epithelium, platelets, cardiac and skeletal muscle. PLC-delta 3 preferentially hydrolyzes membrane PIP2. This generates IP3, which induces intracellular Ca++ release, and DAG, activating PKC. Human PLC-delta 3 is 789 amino acids (aa) in length. It contains a plextrin homology domain that tethers the enzyme to the membrane (aa 63‑172), three EF-hand motifs that spatially orient the molecule (aa 182‑285), a catalytic region (aa 337‑644), and a C2 domain that secures the catalytic region to its substrate (aa 647‑752). There are multiple potential isoforms. There is an alternate start site at Met54, a 40 aa substitution for the C-terminal 248 aa, and a Leu substitution for aa 102‑109. Over aa 148‑344, human PLC-delta 3 is 93% aa identical to mouse PLC-delta 3.
Long Name
Phospholipase C delta 3
Alternate Names
PLCD3, PLCdelta 3
Gene Symbol
PLCD3
UniProt
Additional PLC-delta 3 Products
Product Documents for Human/Mouse PLC-delta3 Antibody
Product Specific Notices for Human/Mouse PLC-delta3 Antibody
For research use only
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