Human/Mouse RASSF2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5639
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RASSF2
Lys100-Gly230
Accession # P50749
Lys100-Gly230
Accession # P50749
Specificity
Detects endogenous human and mouse RASSF2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse RASSF2 Antibody
Detection of Human RASSF2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and Daudi human Burkitt's lymphoma cell line . PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse RASSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RASSF2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RASSF2 in U937 Human Cell Line.
RASSF2 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human/Mouse RASSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5639) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse RASSF2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed U937 human histiolytic lymphoma cell line
Sample: Immersion fixed U937 human histiolytic lymphoma cell line
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and Daudi human Burkitt's lymphoma cell line
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and Daudi human Burkitt's lymphoma cell line
Reviewed Applications
Read 1 review rated 5 using AF5639 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RASSF2
Long Name
Ras Association [RalGDS/AF-6] Domain Family Member 2
Alternate Names
RASFADIN
Gene Symbol
RASSF2
UniProt
Additional RASSF2 Products
Product Documents for Human/Mouse RASSF2 Antibody
Product Specific Notices for Human/Mouse RASSF2 Antibody
For research use only
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