Human/Mouse/Rat AMPK beta1 Antibody

Name: Human/Mouse/Rat AMPK beta1 Antibody
Brand: R&D Systems
SKU: AF2854
Rating: 4 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # AF2854

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Citations (4)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all AMPK beta 1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AMPK beta1
Met1-Ile270
Accession # Q9Y478

Specificity

Detects human, mouse, and rat AMPK beta1 in Western blots. In Western blots, no cross-reactivity with recombinant human AMPK beta2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat AMPK beta1 Antibody

Detection of Human/Mouse/Rat AMPK beta1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat AMPK beta1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human AMPK beta1 and AMPK beta2 (2 ng/lane) were included. A specific band for AMPK beta 1 was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of AMPK beta1 in Human Kidney.

AMPK beta1 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Goat Anti-Human/Mouse/Rat AMPK beta1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of AMPK beta1 in Mouse Kidney.

AMPK beta1 was detected in immersion fixed paraffin-embedded sections of Mouse Kidney using Goat Anti-Human/Mouse/Rat AMPK beta1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Applications for Human/Mouse/Rat AMPK beta1 Antibody

Application

Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Kidney, Mouse Kidney and Rat Kidney

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF2854 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMPK beta 1

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3), with all 12 combinations able to form complexes. The beta1 subunit is expressed at higher levels than the beta2 subunit in liver, while beta2 is more abundant than beta1 in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase complex as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase beta 1

Alternate Names

PRKAB1

Entrez Gene IDs

5564 (Human); 19079 (Mouse); 83803 (Rat)

Gene Symbol