Human/Mouse/Rat Bcl-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2889
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 384202
Product Specifications
Immunogen
E. coli-derived recombinant human Bcl-10
Met1-Leu192
Accession # O95999
Met1-Leu192
Accession # O95999
Specificity
Detects endogenous human, mouse, and rat Bcl-10 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat Bcl-10 Antibody
Detection of Human/Mouse/Rat Bcl-10 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Bcl-10 Monoclonal Antibody (Catalog # MAB2889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl-10 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat Bcl-10 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line
Sample: Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-10
B cell CLL/leukemia 10 (Bcl-10), a 233 amino acid, 26.2 kDa protein, is the cellular homolog of the equine herpesvirus-2 gene (E10). Bcl-10 is also known as CARD like apoptotic protein (CLAP), CED-3/ICH-1 prodomain homologous E10-like regulator (CIPER), celluar-E10 (c-E10), and CARD-containing molecule enhancing NF kappaB (CARMEN). Bcl-10 contains a caspase recruitment domain (CARD) and has been shown to induce apoptosis and to activate NF kappaB. Bcl-10 can interact with other CARD domain containing proteins including CARD9, 10, 11, and 14, which function as upstream regulators of NF kappaB signaling. Defects in Bcl‑10 may be involved in the pathogenesis of human malignancy. Over aa 1-192, human Bcl-10 is 92% and 93% aa identical to mouse and rat Bcl-10, respectively.
Long Name
B Cell Lymphoma/Leukemia 10
Alternate Names
Bcl10, c-E10, CARMEN, CIPER, CLAP, mE10
Entrez Gene IDs
8915 (Human)
Gene Symbol
BCL10
UniProt
Additional Bcl-10 Products
Product Documents for Human/Mouse/Rat Bcl-10 Antibody
Product Specific Notices for Human/Mouse/Rat Bcl-10 Antibody
For research use only
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