Human/Mouse/Rat Calcineurin A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2839
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 302202
Product Specifications
Immunogen
Bovine brain Calcineurin holoenzyme (A and B subunits)
Specificity
Detects human, mouse, and rat Calcineurin A.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat Calcineurin A Antibody
Detection of Human/Mouse/Rat Calcineurin A by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat Calcineurin A Monoclonal Antibody (Catalog # MAB2839) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Calcineurin (Catalog # 3160-CA) (1 ng) was included. A specific band for Calcineurin A was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Calcineurin A Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line
Sample: HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calcineurin A
Calcineurin A, also known as PP2B and PPP3CA, is the 59 kDa catalytic subunit of the calcium/calmodulin-dependent protein phosphatase. When activated by calcium in the presence of the regulatory B subunit and calmodulin, Calcineurin A selectively removes phosphates from serine and threonine residues on target proteins. Although ubiquitously expressed, Calcineurin levels are highest in brain, where the phosphatase plays a role in the formation and retention of memories. Calcineurin activity is inhibited by the immunosuppressant drug cyclosporine A bound to cyclophilins.
Alternate Names
CALNA, CNA1, PP2B, PP3CA, PPP2B, PPP3CA
Gene Symbol
PPP3CA
Additional Calcineurin A Products
Product Documents for Human/Mouse/Rat Calcineurin A Antibody
Product Specific Notices for Human/Mouse/Rat Calcineurin A Antibody
For research use only
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