Human/Mouse/Rat Complexin-1/2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8069

R&D Systems, part of Bio-Techne
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MAB8069
MAB8069-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 837802

View all Complexin-1/2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Complexin-1
Met1-Lys134
Accession # O14810

Specificity

Detects human Complexin-1 and Complexin-2 in ELISAs. Detects human, mouse, and rat Complexin-1 and -2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat Complexin-1/2 Antibody

Detection of Human, Mouse, and Rat Complexin-1/2 antibody by Western Blot.

Detection of Human, Mouse, and Rat Complexin-1/2 by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Complexin-1/2 Monoclonal Antibody (Catalog # MAB8069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Complexin-1/2 at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Complexin-1/2 antibody in Human Pancreas by Immunohistochemistry (IHC-P).

Complexin-1/2 in Human Pancreas.

Complexin-1/2 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse/Rat Complexin-1/2 Monoclonal Antibody (Catalog # MAB8069) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Complexin-1/2 antibody by Simple WesternTM.

Detection of Human Complexin-1/2 by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.5 mg/mL. A specific band was detected for Complexin-1/2 at approximately 23 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Complexin-1/2 Monoclonal Antibody (Catalog # MAB8069). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human/Mouse/Rat Complexin-1/2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Simple Western

5 µg/mL
Sample: Human brain (cerebellum) tissue

Western Blot

0.5 µg/mL
Sample: Human brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complexin-1/2

Complexin-1 and Complexin-2 are cytosolic proteins that belongs to the complexin/synaphin family. They are expressed in select neurons and pancreatic b-cells. Complexin-1 serves as a block against premature neurotransmitter/insulin granule release. It does so by binding to the SNARE complex that contains SNAP25, VAMP2 and STX1A, generating oligomers of SNARE complexes that are optimally oriented relative to the positions of vesicle and plasma membranes. This effectively places synaptic vesicles in an optimal configuration, allowing for a coordinated, uniform fusion and release upon appropriate stimulus. Typically, complexin-1 is described as a clamp that blocks (vesicle) fusion, and the stimulus that overcomes it is one that induces an influx of synaptotagmin-detectable calcium. Both human complexin-1 and complexin-2 are 134 amino acids (aa) in length. Full-length human complexin-1 shares 84% aa sequence identity with human complexin-2. Human and mouse complexin-1 share 97% aa sequence identity, while human and mouse complexin-2 are 100% identical.

Alternate Names

CPLX1;CPX1;CPX-I;DEE63;EIEE63

UniProt

O14810 (Human)

Additional Complexin-1/2 Products

Product Documents for Human/Mouse/Rat Complexin-1/2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Complexin-1/2 Antibody

For research use only

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