Human/Mouse/Rat Fgr Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3207
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 349316
Product Specifications
Immunogen
E. coli-derived recombinant human Fgr
Met1-Phe82
Accession # P09769
Met1-Phe82
Accession # P09769
Specificity
Detects endogenous human, mouse, and rat Fgr in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat Fgr Antibody
Detection of Human and Rat Fgr by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat Fgr Monoclonal Antibody (Catalog # MAB3207) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fgr at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat Fgr Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: NRK rat normal kidney cell line, Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Sample: NRK rat normal kidney cell line, Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB3207 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fgr
Fgr is the cellular homolog of the oncogenic protein encoded by the Gardner-Rasheed feline sarcoma virus. A member of the Src family of non‑receptor tyrosine kinases, Fgr is predominantly expressed in neutrophils, macrophages, monocytes, and natural killer cells. Mice lacking Fgr fail to recruit eosinophils to the lung during allergic airway inflammation.
Long Name
Gardner-Rasheed Feline Sarcoma Viral Oncogene Homolog
Alternate Names
Src2
Gene Symbol
FGR
UniProt
Additional Fgr Products
Product Documents for Human/Mouse/Rat Fgr Antibody
Product Specific Notices for Human/Mouse/Rat Fgr Antibody
For research use only
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