Human/Mouse/Rat Follistatin-like 4/FSTL4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5245
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Follistatin-like 4/FSTL4
Trp23-Val841
Accession # Q5STE3
Trp23-Val841
Accession # Q5STE3
Specificity
Detects recombinant mouse Follistatin-like 4/FSTL4 in direct ELISAs. Detects human, mouse, and rat Follistatin-like 4/FSTL4 in Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FSTL4 is observed, and less than 5% cross‑reactivity with rhFSTL5 and recombinant mouse FSTL1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Follistatin-like 4/FSTL4 Antibody
Detection of Human, Mouse, and Rat Follistatin‑like 4/FSTL4 by Western Blot.
Western blot shows lysates of mouse brain (cortex) tissue, rat brain (cortex) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Follistatin-like 4/FSTL4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Follistatin-like 4/FSTL4 in Mouse Brain.
Follistatin-like 4/FSTL4 was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons in the brainstem. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. This application has not been tested in human or rat samplesApplications for Human/Mouse/Rat Follistatin-like 4/FSTL4 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain
Sample: Perfusion fixed frozen sections of mouse brain
Western Blot
1 µg/mL
Sample: Mouse brain (cortex) tissue, rat brain (cortex) tissue, and human brain (hippocampus) tissue
Sample: Mouse brain (cortex) tissue, rat brain (cortex) tissue, and human brain (hippocampus) tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Follistatin-like 4/FSTL4
FSTL4 (Follistatin-like protein 4; also FSL4, SPIG1 and D/Bsp120I-1) is a likely secreted, 90 kDa (predicted) member of the follistatin gene family of TGF-beta superfamily inhibitors. It is widely expressed, being found in neurons (retinal ganglion and cerebellar Purkinje cells), cardiac muscle cells, smooth muscle cells and intestinal epithelium. Mature mouse FSTL4 is 819 amino acids (aa) in length (aa 23-841). It contains one kazal-like domain (aa 80-134), an EF-hand motif (aa 173-208), and two Ig-like domains (aa 250-336 and 340-425). There are two potential splice forms, one that shows an alternative start site at Met315, and a second that possesses a His residue substitution for aa 518-841. Full-length mature mouse FSTL4 shares 95% and 84% aa identity with rat and human FSTL4, respectively.
Alternate Names
Follistatin like 4, FSL4, FSTL4
Gene Symbol
FSTL4
UniProt
Additional Follistatin-like 4/FSTL4 Products
Product Documents for Human/Mouse/Rat Follistatin-like 4/FSTL4 Antibody
Product Specific Notices for Human/Mouse/Rat Follistatin-like 4/FSTL4 Antibody
For research use only
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