Human/Mouse/Rat FRS2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4069
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 462910
Product Specifications
Immunogen
E. coli-derived recombinant human FRS2
Asn121-Asn449
Accession # Q8WU20
Asn121-Asn449
Accession # Q8WU20
Specificity
Detects human, mouse, and rat FRS2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat FRS2 Antibody
Detection of Human/Mouse/Rat FRS2 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FRS2 Monoclonal Antibody (Catalog # MAB4069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat FRS2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB4069 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FRS2
FRS2 (FGF R substrate 2; also known as SNT and FRS2 alpha) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF R and other RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain (aa 13‑115) that interacts with FGF and NGF receptors, and a C-terminal Tyr-rich region (aa 196‑471) that serves as a docking site for GRB‑2 and SHP‑2.
Long Name
Fibroblast Growth Factor Receptor Substrate 2
Alternate Names
FRS2alpha, SNT1
Gene Symbol
FRS2
UniProt
Additional FRS2 Products
Product Documents for Human/Mouse/Rat FRS2 Antibody
Product Specific Notices for Human/Mouse/Rat FRS2 Antibody
For research use only
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