Human/Mouse/Rat GATE-16 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9035
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 853746
Product Summary for Human/Mouse/Rat GATE-16 Antibody
Immunogen
E.coli-derived recombinant human GATE16
Accession # P60520
Accession # P60520
Specificity
Detects human, mouse, and rat GATE-16 in Western blot.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat GATE-16 Antibody
Detection of Human, Mouse, and Rat GATE-16 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, mouse brain (cerebellum) tissue, and rat brain (total) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat GATE-16 Monoclonal Antibody (Catalog # MAB9035) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for GATE-16 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat GATE-16 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, mouse brain (cerebellum) tissue, and rat brain (total) tissue
Sample: K562 human chronic myelogenous leukemia cell line, mouse brain (cerebellum) tissue, and rat brain (total) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATE-16
Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (Atg8) family of proteins (1). GATE-16/Apg8p2 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16/Apg8p2 is best known for its role in autophagy (2,3). GATE-16/Apg8p2 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).
References
- Shpilka, T. et al. (2011) Genome Biol. 12:226.
- Wang, H. et al. (1999) Nature 397:69.
- Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
- Weidberg, H. et al. (2010) EMBO J. 29:1792.
- Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125
Long Name
Golgi-associated ATPase Enhancer of 16 kDa
Alternate Names
Apg8p2, ATG8C, FLC3A, GABARAPL2, GATE16, GEF2
Gene Symbol
GABARAPL2
UniProt
Additional GATE-16 Products
Product Documents for Human/Mouse/Rat GATE-16 Antibody
Product Specific Notices for Human/Mouse/Rat GATE-16 Antibody
For research use only
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