Human/Mouse/Rat HCLS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5789

R&D Systems, part of Bio-Techne
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AF5789
AF5789-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all HCLS1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HCLS1
Met1-Gly84
Accession # P14317

Specificity

Detects endogenous human, mouse and rat HCLS1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat HCLS1 Antibody

Detection of Human/Mouse/Rat HCLS1 antibody by Western Blot.

Detection of Human/Mouse/Rat HCLS1 by Western Blot.

Western blot shows lysates of L1.2 mouse pro-B cell line, Nb2-11 rat lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat HCLS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5789) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HCLS1 at approximately 75 to 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat HCLS1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: L1.2 mouse pro-B cell line, Nb2-11 rat lymphoma cell line, and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HCLS1

HCLS1 (Hematopoietic lineage cell-specific protein; also HS1 and LckBP1) is a cytoplasmic substrate for non‑receptor type protein‑tyrosine kinases. Although its predicted MW is 54 kDa, it migrates anomalously at 75‑80 kDa in SDS-PAGE. HCLS1 is restricted to myeloid/lymphoid lineage cell types, including platelets, B cells, T cells and mast cells. HCLS1 binds to F-actin and promotes actin polymerization. It also serves as an intermediate for src-mediated signal transduction. Human HCLS1 is 486 amino acids (aa) in length. It contains one HAX1 binding site (aa 27‑66), three-plus cortactin type repeats (aa 79‑212), a coiled‑coil region (aa 215‑280) and an SH3 domain (aa 437‑484). HCLS1 is initially phosphorylated by Syk at Tyr378 and Tyr397, followed by a Src family kinase phosphorylation at Tyr222. This activates HCLS1, allowing it to interact with Ras-associated Grb2.

Long Name

Hematopoietic Cell-specific Lyn Substrate 1

Alternate Names

Cortactin-like, CTTNL, LckBP1

Entrez Gene IDs

3059 (Human); 15163 (Mouse); 288077 (Rat)

Gene Symbol

HCLS1

UniProt

P14317

Additional HCLS1 Products

Product Documents for Human/Mouse/Rat HCLS1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat HCLS1 Antibody

For research use only

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