Human/Mouse/Rat Hepassocin/FGL1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1614
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry, Simple Western, Western Blot
Cited:
Mass Spectrometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690
Met1-Ile312
Accession # BAB70690
Specificity
Detects human Hepassocin/FGL1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Hepassocin/FGL1 Antibody
Detection of Human, Mouse, and Rat Hepassocin/FGL1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/ Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Hepassocin/FGL1 homodimer at approximately 65 kDa (as indicated). Hepassocin/FGL1 is resistant to reducing conditions (1). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Hepassocin/FGL1 in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF1614, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Intracellular Molecules.
Detection of Human Hepassocin/FGL1 by Simple WesternTM.
Simple Western lane view shows lysates of Hep3B human hepatocellular carcinoma cell line, human liver, loaded at 0.2 mg/mL. A specific band was detected for Hepassocin/FGL1 at approximately 41 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat Hepassocin/FGL1 Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: HepG2 Human cell line
Sample: HepG2 Human cell line
Simple Western
20 µg/mL
Sample: Hep3B human hepatocellular carcinoma cell line, human liver
Sample: Hep3B human hepatocellular carcinoma cell line, human liver
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, Hep3B human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, Hep3B human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hepassocin/FGL1
References
- 1.Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
Long Name
Fibrinogen Like 1
Alternate Names
FGL1, Fibrinogen-like-protein 1, FREP1
Gene Symbol
FGL1
UniProt
Additional Hepassocin/FGL1 Products
Product Documents for Human/Mouse/Rat Hepassocin/FGL1 Antibody
Product Specific Notices for Human/Mouse/Rat Hepassocin/FGL1 Antibody
For research use only
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