Human/Mouse/Rat HSP70/HSPA1A Antibody

R&D Systems, part of Bio-Techne | Catalog # A-400

R&D Systems, part of Bio-Techne
Discontinued Product
A-400 has been discontinued. View all HSP70/HSPA1A products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 242707

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSP70/HSPA1A
Met1-Asp641

Specificity

Detects the induced form of human and mouse HSP70/HSPA1A in Western blots. No cross reactivity with HSC70 (HSP73) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat HSP70/HSPA1A Antibody

Detection of Human HSP70/HSPA1A antibody by Western Blot.

Detection of Human HSP70/HSPA1A by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # A-400) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP70/HSPA1A at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat HSP70/HSPA1A Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL

Western Blot

0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: HSP70/HSPA1A

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions, but are dramatically up-regulated in response to cellular stress. HSP70 (also known as HSPA1A, HSP70-1, and HSP72) is a 72 kDa member of the heat shock protein 70 family of proteins. HSP70 is required to target some misfolded proteins to the Ubiquitin E3 ligase CHIP (also known as Stub1) for subsequent ubiquitination.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Entrez Gene IDs

3303 (Human); 15511 (Mouse); 24472 (Rat)

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for Human/Mouse/Rat HSP70/HSPA1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat HSP70/HSPA1A Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

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