Human/Mouse/Rat LHX5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6290
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human LHX5
Leu265-Trp402
Accession # Q9H2C1
Leu265-Trp402
Accession # Q9H2C1
Specificity
Detects recombinant human LHX5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LIM1 is observed, and less than 5% cross-reactivity with rhLHX2, and rhLHX9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat LHX5 Antibody
Detection of Rat LHX5 by Western Blot.
Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LHX5 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LHX5 in Mouse Brain.
LHX5 was detected in perfusion fixed frozen sections of adult mouse brain (hippocampus) using Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse/Rat LHX5 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of adult mouse brain (hippocampus)
Sample: Perfusion fixed frozen sections of adult mouse brain (hippocampus)
Western Blot
1 µg/mL
Sample: Rat embryonic hippocampal neurons
Sample: Rat embryonic hippocampal neurons
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LHX5
Long Name
LIM (Lin11:Isl1:Mec3)/Homeobox Protein 5
Alternate Names
LIM homeobox 5, LIM homeobox protein 5, LIM/homeobox protein Lhx5, MGC129689
Gene Symbol
LHX5
UniProt
Additional LHX5 Products
Product Documents for Human/Mouse/Rat LHX5 Antibody
Product Specific Notices for Human/Mouse/Rat LHX5 Antibody
For research use only
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