Human/Mouse/Rat MP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4367
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 448406
Product Specifications
Immunogen
E. coli-derived recombinant human MP1
Ala2-Ser124
Accession # Q9UHA4
Ala2-Ser124
Accession # Q9UHA4
Specificity
Detects human, mouse, and rat MP1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat MP1 Antibody
Detection of Human/Mouse/Rat MP1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, Balb/3T3 mouse embryonic fibroblast cell line, NRK rat normal kidney cells, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat MP1 Monoclonal Antibody (Catalog # MAB4367) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MP1 (2 ng) was included. A specific band for MP1 was detected at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat MP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, Balb/3T3 mouse embryonic fibroblast cell line, NRK rat normal kidney cells, and PC-12 rat adrenal pheochromocytoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, Balb/3T3 mouse embryonic fibroblast cell line, NRK rat normal kidney cells, and PC-12 rat adrenal pheochromocytoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MP1
MP1 (MEK Partner 1), gene name LAMTOR3 (late endosomal/lysosomal adaptor and MAPK and MTOR activator 3), was initially identified as a scaffold protein that tethers MEK1 and ERK1 to facilitate their activation. MP1 and other MAPK scaffolds promote phosphorylation efficiency and specificity, localize their bound pathway components to particular subcellular sites, and serve as nodes of signal integration for regulation of MAPK pathways by other signaling events. With the assistance of adaptor protein p14, the bound MP1 complex localizes to late endosomes and is thought to regulate endosomal traffic and cellular proliferation during tissue homeostasis.
Long Name
MEK-binding Protein 1
Alternate Names
LAMTOR3, MAP2K1IP1, Mapbp
Gene Symbol
LAMTOR3
UniProt
Additional MP1 Products
Product Documents for Human/Mouse/Rat MP1 Antibody
Product Specific Notices for Human/Mouse/Rat MP1 Antibody
For research use only
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