Human/Mouse/Rat MST2/STK3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4549
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2072A
Product Specifications
Immunogen
E. coli-derived recombinant human STK3
Ala339-Asp456
Accession # Q13188
Ala339-Asp456
Accession # Q13188
Specificity
Detects human STK3 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat MST2/STK3 Antibody
Detection of Human, Mouse, and Rat MST2/STK3 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, C6 rat glioma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat MST2/STK3 Monoclonal Antibody (Catalog # MAB4549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MST2/STK3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MST2/STK3 in A431 Human Cell Line.
MST2/STK3 was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human MST2/STK3 Monoclonal Antibody (Catalog # MAB4549) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MST2/STK3 in Human Kidney.
MST2/STK3 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human/Mouse/Rat MST2/STK3 Monoclonal Antibody (Catalog # MAB4549) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in epithelial cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse/Rat MST2/STK3 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Immunohistochemistry
0.3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Simple Western
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line
Western Blot
0.1 µg/mL
Sample: A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, C6 rat glioma cell line, and Ramos human Burkitt's lymphoma cell line
Sample: A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, C6 rat glioma cell line, and Ramos human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MST2/STK3
MST2 (mammalian sterile twenty-like 2; also known as STK3 and Krs-1) is a 58 kDa member of the GCKII group of the STE20 subfamily of the STE Ser/Thr kinase protein family. In human, MST2 is 491 amino acids (aa) in length. It contains one kinase domain (aa 27‑278), two NESs (aa 361‑371 and 438‑447) and coiled-coil domains (aa 287‑328 and 442‑475), and one NLS (aa 473‑487). A caspase-cleavage site exists between Asp322‑Ser323. MST2 is normally inactive in the cytoplasm bound to Raf-1. Cell activation induces Raf-1:MST2 dissociation, MST2 cleavage, and N-terminal translocation to the nucleus. Human MST2 is 99% and 96% aa identical to canine and mouse MST2, respectively.
Alternate Names
KRS1, Mess1, MST3, STK3
Gene Symbol
STK3
UniProt
Additional MST2/STK3 Products
Product Documents for Human/Mouse/Rat MST2/STK3 Antibody
Product Specific Notices for Human/Mouse/Rat MST2/STK3 Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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