Human/Mouse/Rat Musashi-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2628
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Musashi-1
Met1-His362
Accession # O43347
Met1-His362
Accession # O43347
Specificity
Detects human Musashi-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Musashi‑2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Musashi-1 Antibody
Detection of Recombinant Human Musashi‑1 by Western Blot.
Western blot shows 25 ng of Recombinant Human Musashi-1 and Recombinant Human Musashi-2. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/ Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Musashi-1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Musashi‑1 in Mouse Cortical Stem Cells.
Musashi-1 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Musashi‑1 in Rat Cortical Stem Cells.
Musashi-1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human/Mouse/Rat Musashi-1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed undifferentiated rat and mouse cortical stem cells
Sample: Immersion fixed undifferentiated rat and mouse cortical stem cells
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human intestine
Sample: Immersion fixed paraffin-embedded sections of human intestine
Western Blot
0.1 µg/mL
Sample: Recombinant Human Musashi-1
Sample: Recombinant Human Musashi-1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Musashi-1
References
- Kaneko, Y. et al. (2000) Dev. Neurosci. 22:139.
- Kanemura, Y. et al. (2002) Meth. Mol. Biol. 198:273.
Alternate Names
MSI1, Musashi1
Entrez Gene IDs
4440 (Human)
Gene Symbol
MSI1
UniProt
Additional Musashi-1 Products
Product Documents for Human/Mouse/Rat Musashi-1 Antibody
Product Specific Notices for Human/Mouse/Rat Musashi-1 Antibody
For research use only
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