Human/Mouse/Rat nNOS Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2416
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Saimiri sciureus (Common Squirrel Monkey)
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human nNOS
Ser218-Ser1434
Accession # P29475
Ser218-Ser1434
Accession # P29475
Specificity
Detects human, mouse, and rat nNOS in Western blots. In direct ELISAs, this antibody shows approximately 5% cross‑reactivity with recombinant human eNOS.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat nNOS Antibody
Detection of Mouse and Rat nNOS by Western Blot.
Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for nNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
nNOS in Human Brain.
nNOS was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 1.7 µg/mL Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of astrocytes in the cortex. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
nNOS in Rat Brain.
nNOS was detected in immersion fixed frozen sections of rat brain (cortex) using Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons and neuronal processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse/Rat nNOS Antibody
Application
Recommended Usage
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) and immersion fixed frozen sections of rat brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) and immersion fixed frozen sections of rat brain (cortex)
Simple Western
10 µg/mL
Sample: Mouse brain tissue and rat brain tissue
Sample: Mouse brain tissue and rat brain tissue
Western Blot
1 µg/mL
Sample: Mouse brain tissue and rat brain tissue
Sample: Mouse brain tissue and rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: nNOS
Long Name
Neuronal Nitric Oxide Synthase
Alternate Names
NOS1
Gene Symbol
NOS1
UniProt
Additional nNOS Products
Product Documents for Human/Mouse/Rat nNOS Antibody
Product Specific Notices for Human/Mouse/Rat nNOS Antibody
For research use only
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