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Human/Mouse/Rat nNOS Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2416

R&D Systems, part of Bio-Techne
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AF2416
AF2416-SP

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Saimiri sciureus (Common Squirrel Monkey)

Applications

Validated:

Immunohistochemistry, Simple Western, Western Blot

Cited:

Immunohistochemistry, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human nNOS
Ser218-Ser1434
Accession # P29475

Specificity

Detects human, mouse, and rat nNOS in Western blots. In direct ELISAs, this antibody shows approximately 5% cross‑reactivity with recombinant human eNOS.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat nNOS Antibody

Detection of Mouse and Rat nNOS antibody by Western Blot.

Detection of Mouse and Rat nNOS by Western Blot.

Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for nNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
nNOS antibody in Human Brain by Immunohistochemistry (IHC-P).

nNOS in Human Brain.

nNOS was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 1.7 µg/mL Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of astrocytes in the cortex. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
nNOS antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

nNOS in Rat Brain.

nNOS was detected in immersion fixed frozen sections of rat brain (cortex) using Goat Anti-Human/Mouse/Rat nNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2416) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons and neuronal processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat nNOS Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) and immersion fixed frozen sections of rat brain (cortex)

Simple Western

10 µg/mL
Sample: Mouse brain tissue and rat brain tissue

Western Blot

1 µg/mL
Sample:   Mouse brain tissue and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: nNOS

nNOS is one of three NOS enzymes that catalyze the oxidation of L-argine to L-citruline and nitric oxide. nNOS exists as homodimers containing a cytochrome P450‑like prosthetic heme group in the N-terminal half. It also has a tightly bound FAD and FMN group in the C-terminal half. At least 4 isoforms of human nNOS are known. Human nNOS shares about 55% amino acid sequence identity with eNOS and iNOS. It also shares 96% sequence identity with mouse or rat nNOS.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Entrez Gene IDs

4842 (Human); 18125 (Mouse)

Gene Symbol

NOS1

UniProt

Additional nNOS Products

Product Documents for Human/Mouse/Rat nNOS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat nNOS Antibody

For research use only

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