Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Western Blot

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 215247

View all p70 S6 Kinase Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p70 S6 Kinase
Accession # M60725

Specificity

Detects human, mouse, and rat p70 S6 Kinase and p85 S6 Kinase in Western blots. Reactivity with beta isoforms of p70 S6 Kinase is unknown.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat p70 S6 Kinase PE-conjugated Antibody

Detection of p70 S6 Kinase antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of p70 S6 Kinase in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat p70 S6 Kinase PE-conjugated Monoclonal Antibody (Catalog # IC8962P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse/Rat p70 S6 Kinase PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: p70 S6 Kinase

p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes during the cell cycle and increases 20‑fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well asT389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK1) (1-3). Over amino acids (aa) 1-502, human and mouse share 99% aa sequence identity.

References

  1. Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
  2. Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
  3. Fenton, T.R. and  I.T. Gout (2011) Int. J. Biochem. Cell Biol. 43:47.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Entrez Gene IDs

6198 (Human); 72508 (Mouse); 83840 (Rat)

Gene Symbol

RPS6KB1

UniProt

M60725

Additional p70 S6 Kinase Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat p70 S6 Kinase PE-conjugated Antibody

For research use only

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