Human/Mouse/Rat PAK6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4265
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PAK6
Met1-Arg137
Accession # Q9NQU5
Met1-Arg137
Accession # Q9NQU5
Specificity
Detects endogenous human, mouse and rat PAK6 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PAK6 Antibody
Detection of Human/Mouse/Rat PAK6 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A431 human epithelial carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat PAK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PAK6 at approximately 75 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat PAK6 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line, A431 human epithelial carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line
Sample: HT-29 human colon adenocarcinoma cell line, A431 human epithelial carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAK6
As downstream targets of the Rho GTPases, the p21-activated kinase (PAK) family of serine/threonine kinases regulates the organization of the actin cytoskeleton in mammalian cells. The PAK family is structurally categorized in two groups, each with three members: group I PAK1-3; and group II PAK4-6. PAK6 is expressed most highly in brain and testes, with lower levels in multiple tissues. Both MKK6 and p38 MAPK activate PAK6, suggesting a role for this kinase in the cellular stress response.
Long Name
p21/Cdc42/Rac1-activated Kinase 6
Alternate Names
EC 2.7.11, p21 protein (Cdc42/Rac)-activated kinase 6, p21(CDKN1A)-activated kinase 6, p21-activated kinase 6, p21-activated protein kinase 6, PAK-5, PAK5EC 2.7.11.1, PAK-6, serine/threonine-protein kinase PAK 6
Gene Symbol
PAK6
UniProt
Additional PAK6 Products
Product Documents for Human/Mouse/Rat PAK6 Antibody
Product Specific Notices for Human/Mouse/Rat PAK6 Antibody
For research use only
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