Human/Mouse/Rat Peroxiredoxin 5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5724

R&D Systems, part of Bio-Techne
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AF5724
AF5724-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Peroxiredoxin 5 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Peroxiredoxin 5
Met1-Leu210
Accession # P99029

Specificity

Detects human, mouse and rat Peroxiredoxin 5 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Peroxiredoxin 5 Antibody

Detection of Human/Mouse/Rat Peroxiredoxin 5 antibody by Western Blot.

Detection of Human/Mouse/Rat Peroxiredoxin 5 by Western Blot.

Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Peroxiredoxin 5 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

Peroxiredoxin 5 in A549 Human Cell Line.

Peroxiredoxin 5 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Peroxiredoxin 5 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

Peroxiredoxin 5 in A549 Human Cell Line.

Peroxiredoxin 5 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (mitochondria). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Peroxiredoxin 5 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Western Blot

1 µg/mL
Sample: Human, mouse,and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peroxiredoxin 5

Peroxiredoxin 5 (Prx-5; also known as AOEB166 and thioredoxin reductase) is a 22 kDa, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The mouse precursor molecule is 210 amino acids (aa) in length and contains an N-terminal 48 aa mitochondrial targeting (signal) sequence and a 162 aa mature enzyme that shows an NES between aa 54‑63, and a peroxisome targeting motif at aa 208‑210. There are two catalytic cysteines, one at Cys96, and another at Cys200 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H2O2 and alkyl hydroperoxides. Two potential splice forms are reported. One is 17 kDa in size and shows an alternative start site at Met49, while a second shows a 19 aa substitution for aa 83‑102. Mouse Prx-5 is 78% and 90% aa identical to human and rat Prx-5, respectively.

Alternate Names

ACR1, AOEB166, AOPP, PMP20, PRDX5, PRXV

Entrez Gene IDs

25824 (Human); 54683 (Mouse); 113898 (Rat)

Gene Symbol

PRDX5

UniProt

P99029

Additional Peroxiredoxin 5 Products

Product Documents for Human/Mouse/Rat Peroxiredoxin 5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Peroxiredoxin 5 Antibody

For research use only

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