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Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5126

R&D Systems, part of Bio-Techne
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AF5126
AF5126-SP

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Hamster - Cricetulus (Chinese Hamster)

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Product Specifications

Immunogen

Phosphopeptide containing human FRS2 Y436 site

Specificity

Detects human, mouse, and rat FRS2 isoforms when phosphorylated at Y436.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

Detection of Human and Rat Phospho-FRS2 (Y436) antibody by Western Blot.

Detection of Human and Rat Phospho-FRS2 (Y436) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta-NGF (Catalog # 556-NG) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Bands were detected for Phospho-FRS2 (Y436) at approximately 70 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-FRS2 (Y436) antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

Phospho-FRS2 (Y436) in A431 Human Cell Line.

FRS2 phosphorylated at Y436 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with pervanadate

Western Blot

1 µg/mL
Sample: Pervanadate-treated MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line treated with Recombinant Rat beta-NGF (Catalog # 556-NG)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRS2

FRS2 (FGF R substrate 2; also known as SNT and FRS2-alpha) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF and RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain that interacts with FGF and NGF receptors (aa 13‑115), and a C-terminal tyrosine-rich region that serves as a docking site for GRB-2 and SHP-2 (aa 196‑471). Phosphorylation of Y436 by activated RTKs is required for efficient SHP-2 recruitment.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Alternate Names

FRS2alpha, SNT1

Entrez Gene IDs

10818 (Human); 327826 (Mouse)

Gene Symbol

FRS2

Additional FRS2 Products

Product Documents for Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

For research use only

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