Human/Mouse/Rat PINCH1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6257
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Fish - Danio rerio (Zebrafish)
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 591604
Product Specifications
Immunogen
E. coli-derived recombinant human PINCH1
Ala2-Gly108
Accession # P48059
Ala2-Gly108
Accession # P48059
Specificity
Detects human, mouse, and rat PINCH1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat PINCH1 Antibody
Detection of Human and Rat PINCH1 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, WI-38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PINCH1 Monoclonal Antibody (Catalog # MAB6257) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PINCH1 at approximately 37-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat PINCH1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line, WI‑38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line
Sample: A549 human lung carcinoma cell line, WI‑38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PINCH1
Particularly interesting new Cys-His protein 1 (PINCH1), also known as LIMS1 (LIM and senescent cell antigen-like-containing domain protein 1), is a 37‑40 kDa, widely expressed LIM-only protein. Human PINCH1 is 325 amino acids (aa) in length and contains a tandem array of five LIM domains. Within the region used as an immunogen, human PINCH1 shares 100% and 99% aa sequence identity with mouse and rat PINCH1, respectively. PINCH1 is an effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation.
Long Name
Particularly Interesting New Cys-His Protein 1
Alternate Names
LIMS1
Entrez Gene IDs
3987 (Human)
Gene Symbol
LIMS1
UniProt
Additional PINCH1 Products
Product Documents for Human/Mouse/Rat PINCH1 Antibody
Product Specific Notices for Human/Mouse/Rat PINCH1 Antibody
For research use only
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