Human/Mouse/Rat PKLR Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8519
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PKLR
Leu47-Ser574
Accession # P30613
Leu47-Ser574
Accession # P30613
Specificity
Detects human, mouse, and rat PKLR in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human PKM-2 is observed.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PKLR Antibody
Detection of Human, Mouse, and Rat PKLR by Western Blot.
Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat PKLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8519) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKLR at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse PKLR by Simple WesternTM.
Simple Western lane view shows lysates of human liver and mouse liver, loaded at 0.2 mg/mL. A specific band was detected for PKLR at approximately 57 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat PKLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8519) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat PKLR Antibody
Application
Recommended Usage
Simple Western
0.5 µg/mL
Sample: Human Liver and Mouse Liver
Sample: Human Liver and Mouse Liver
Western Blot
1:1000 dilution
Sample: Human liver tissue, mouse liver tissue, and rat liver tissue
Sample: Human liver tissue, mouse liver tissue, and rat liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: PKLR
Long Name
Pyruvate Kinase, Liver And RBC
Alternate Names
PK1, PKL, Pyruvate Kinase 1, RPK
Gene Symbol
PKLR
UniProt
Additional PKLR Products
Product Documents for Human/Mouse/Rat PKLR Antibody
Product Specific Notices for Human/Mouse/Rat PKLR Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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