Human/Mouse/Rat PNUTS Antibody

Name: Human/Mouse/Rat PNUTS Antibody
Brand: R&D Systems
SKU: AF21581

R&D Systems, part of Bio-Techne | Catalog # AF21581

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all PNUTS Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PNUTS
Pro5-Asn226
Accession # NP_002705

Specificity

Detects human, mouse, and rat PNUTS in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PNUTS Antibody

Detection of Mouse and Rat PNUTS by Western Blot.

Western blot shows lysates of TS1 mouse helper T cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat PNUTS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21581) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PNUTS at approximately 114 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Detection of Human PNUTS by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for PNUTS at approximately 100-150 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat PNUTS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21581). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat PNUTS Antibody

Application

Recommended Usage

Simple Western

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

0.1 µg/mL
Sample: TS1 mouse helper T cell line and Y3-Ag rat myeloid cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PNUTS

PP1 nuclear targeting subunit (PNUTS), also known as PPP1R10 and FB19, is a 99 kDa protein that binds the serine/threonine protein phosphatase PP1 and causes its localization to chromatin in the nucleus. C-terminal RGG repeats in PNUTS suggest it may bind to single stranded RNA. The PP1/PNUTS complex may also play a role in regulating the cell cycle-dependent phosphorylation of the oncogene Rb.

Long Name

Phosphatase-1 Nuclear Targeting Subunit

Alternate Names

PPP1R10

Entrez Gene IDs

5514 (Human); 52040 (Mouse); 65045 (Rat)

Gene Symbol