Human/Mouse/Rat PP2C epsilon/PPM1L Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6914
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 700612
Product Specifications
Immunogen
E. coli-derived recombinant human PP2C epsilon/PPPM1L
Asp43-Arg178
Accession # Q5SGD2
Asp43-Arg178
Accession # Q5SGD2
Specificity
Detects human PP2C epsilon/PPPM1L in direct ELISAS and Western blots.
In direct ELISAs and Western blots, no
cross-reactivity with recombinant human PP2C is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat PP2C epsilon/PPM1L Antibody
Detection of Human, Mouse, and Rat PP2C epsilon/PPM1L by Western Blot.
Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Nb2-11 rat lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PP2C e/PPM1L Monoclonal Antibody (Catalog # MAB6914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C e/PPM1L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PP2C epsilon/PPM1L Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: BJAB human Burkitt's lymphoma cell line, Nb2‑11 rat lymphoma cell line, Neuro‑2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line
Sample: BJAB human Burkitt's lymphoma cell line, Nb2‑11 rat lymphoma cell line, Neuro‑2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2C epsilon/PPM1L
Long Name
Protein Phosphatase 2C alpha
Alternate Names
PP2CE, PPM1L
Gene Symbol
PPM1L
UniProt
Additional PP2C epsilon/PPM1L Products
Product Documents for Human/Mouse/Rat PP2C epsilon/PPM1L Antibody
Product Specific Notices for Human/Mouse/Rat PP2C epsilon/PPM1L Antibody
For research use only
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