Human/Mouse/Rat PTP-MEG2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2668
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PTP-MEG2
Met1-Gln593
Accession # P43378
Met1-Gln593
Accession # P43378
Specificity
Detects human, mouse, and rat PTP-MEG2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PTP-MEG2 Antibody
Detection of Human, Mouse, and Rat PTP-MEG2 by Western Blot.
Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTP-MEG2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2668) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP-MEG2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PTP-MEG2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEL 92.1.7 human erythroleukemic cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line
Sample: HEL 92.1.7 human erythroleukemic cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP-MEG2
Megakaryocyte protein tyrosine phosphatase 2 (PTP-MEG2), also known as PTPN9, contains an N-terminal domain homologous to the yeast retinaldehyde binding domain Sec14p and a C-terminal phosphatase domain. The Sec14p domain targets PTP-MEG2 to the membrane of secretory vesicles, where it promotes vesicle fusion. Elevated levels of membrane bound PTP-MEG2 activity in erythroid progenitor cells are observed in polycythemia vera and may affect responsiveness to colony stimulating factors.
Long Name
Megakaryocyte Protein Tyrosine Phosphatase 2
Alternate Names
PTPMEG2, PTPN9
Gene Symbol
PTPN9
UniProt
Additional PTP-MEG2 Products
Product Documents for Human/Mouse/Rat PTP-MEG2 Antibody
Product Specific Notices for Human/Mouse/Rat PTP-MEG2 Antibody
For research use only
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