Human/Mouse/Rat RNF2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6065
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RNF2
aa 147-228
Accession # Q99496
aa 147-228
Accession # Q99496
Specificity
Detects human, mouse, and rat RNF2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat RNF2 Antibody
Detection of Human, Mouse, and Rat RNF2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Hepa 1-6 mouse hepatoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RNF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RNF2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat RNF2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Hepa 1‑6 mouse hepatoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: Jurkat human acute T cell leukemia cell line, Hepa 1‑6 mouse hepatoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RNF2
RNF2 (RING [really interesting gene] finger protein 2/1B; also BAP-1, HIP-2 interacting protein 3 and DinG) is a 37 kDa member of the ring finger domain family of proteins. It is expressed in embryonic tissue, and serves as an E3 ubiquitin ligase within a polycomb complex that acts on histone H2A. In effect, RNF2 contributes to gene repression by blocking RNA pol II advance along gene promoters. RNF2 forms homodimers, and interacts with over a dozen proteins. Human RNF2 is 336 amino acids (aa) in length contains one Zn‑finger domain (aa 51‑91) and two phosphorylation sites at Ser168 and Ser203. There is one potential isoform that shows a deletion of aa 84‑155. Over aa 147‑228, human RNF2 shares 99% aa identity with mouse RNF2.
Long Name
Ring Finger Protein 2
Alternate Names
DING, HIPI3, RING1B, RING2
Gene Symbol
RNF2
UniProt
Additional RNF2 Products
Product Documents for Human/Mouse/Rat RNF2 Antibody
Product Specific Notices for Human/Mouse/Rat RNF2 Antibody
For research use only
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