Human/Mouse/Rat SHP-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1878

R&D Systems, part of Bio-Techne
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AF1878
AF1878-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all SHP-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SHP-1
Ala205-Lys595
Accession # P29350

Specificity

Detects human, mouse, and rat SHP-1. The antibody does not detect SHP-2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat SHP-1 Antibody

Detection of Human/Mouse/Rat SHP-1 antibody by Western Blot.

Detection of Human/Mouse/Rat SHP‑1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat SHP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1878) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active SHP-1 aa 205-595 (Catalog # 1878-SH) and Recombinant Human SHP-2 (Catalog # 1894-SH) (2 ng/lane) were included. A specific band for SHP-1 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SHP-1 antibody by Simple WesternTM.

Detection of Human SHP‑1 by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SHP-1 at approximately 65 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat SHP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1878) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat SHP-1 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Nb2-11 rat lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHP-1

Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and "motheaten viable” phenotypes that are severely autoimmune and immunodeficient (1). The enzyme is highly expressed in leukocyte cell types (2). SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors (3). Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity (4). SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor (5). A phosphopeptide containing the EGFR (Y992) sequence (R&D Systems, Catalog # ES006) can be used to measure the activity of SHP-1 by detecting the release of phosphate (R&D Systems, Catalog # DY996).

References

  1. Tsui, H.W. et al. (1993) Nature Genet. 4:124.
  2. Matthews, R.J. et al. (1992) Mol. Cell. Biol. 12:2396.
  3. Burshtyn, D.N. et al. (1997) J. Biol. Chem. 272:13066.
  4. Pei, D. et al. (1994) Biochemistry 33:15483.
  5. Tomic, S. et al. (1995) J. Biol. Chem. 270:21277.

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Entrez Gene IDs

5777 (Human); 15170 (Mouse); 116689 (Rat)

Gene Symbol

PTPN6

UniProt

P29350

Additional SHP-1 Products

Product Documents for Human/Mouse/Rat SHP-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat SHP-1 Antibody

For research use only

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