Human/Mouse/Rat Talin1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5456
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Talin1
Ala975-Ser1181
Accession # Q9Y490
Ala975-Ser1181
Accession # Q9Y490
Specificity
Detects human, mouse and rat Talin1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Talin1 Antibody
Detection of Human/Mouse/Rat Talin1 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Talin1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5456) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Talin1 at approximately 250-270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat Talin1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and NRK rat normal kidney cells
Sample: Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and NRK rat normal kidney cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Talin1
Talin1 (talus; Latin for ankle, the shape of a focal adhesion plaque complex) is a 250‑270 kDa member of the I/LWEQ module superfamily. When activated, it serves as a cytoplasmic, homodimeric link between integrins and vinculin, an actin organizer. Human Talin1 is 2541 amino acids (aa) in length and contains one globular N‑terminal FERM domain that binds integrin cytodomains (aa 86‑403), and a rod shaped C‑terminus (aa 482‑2541) that binds vinculin, and contains an I/LWEQ dimerization domain (aa 2293‑2533). Over amino acids 975‑1181, human Talin1 shares 98% aa identity with mouse Talin1.
Alternate Names
KIAA1027, TLN1
Gene Symbol
TLN1
UniProt
Additional Talin1 Products
Product Documents for Human/Mouse/Rat Talin1 Antibody
Product Specific Notices for Human/Mouse/Rat Talin1 Antibody
For research use only
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