Human/Mouse/Rat Thioredoxin-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5765
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 543317
Product Specifications
Immunogen
E. coli-derived recombinant human Thioredoxin-2
Thr60-Gly166
Accession # Q99757
Thr60-Gly166
Accession # Q99757
Specificity
Detects human, mouse and rat Thioredoxin-2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat Thioredoxin-2 Antibody
Detection of Human/Mouse/Rat Thioredoxin-2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, CH-1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin-2 Monoclonal Antibody (Catalog # MAB5765) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Rat Thioredoxin‑2 by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Thioredoxin‑2 at approximately 11 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Thioredoxin‑2 Monoclonal Antibody (Catalog # MAB5765). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Applications for Human/Mouse/Rat Thioredoxin-2 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and L6 rat myoblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line and L6 rat myoblast cell line
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, CH-1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, CH-1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thioredoxin-2
Alternate Names
MT-TRX, Thioredoxin2, Trx2, TXN2
Gene Symbol
TXN2
UniProt
Additional Thioredoxin-2 Products
Product Documents for Human/Mouse/Rat Thioredoxin-2 Antibody
Product Specific Notices for Human/Mouse/Rat Thioredoxin-2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...