Human/Mouse/Rat/Yeast Rad23 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4555
Key Product Details
Species Reactivity
Human, Mouse, Rat, Yeast
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant yeast Rad23
Accession # P32628
Accession # P32628
Specificity
Detects human, mouse, rat, and yeast Rad23 protein in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat/Yeast Rad23 Antibody
Detection of Human/Mouse/Rat/ Yeast Rad23 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, Nb2-11 rat lymphoma cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Yeast Rad23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4555) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Rad23 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat/Yeast Rad23 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, Nb2-11 rat lymphoma cell line, and mouse brain tissue
Sample: HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, Nb2-11 rat lymphoma cell line, and mouse brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rad23
Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair. All eukaryotic Rad23 proteins contain an amino-terminal UbL domain that bears a striking resemblance to ubiquitin. Rad23 has a proteolytic function in DNA repair via its interaction with the 26S proteosome through the UbL domain. The elimination of this domain causes sensitivity to ultraviolet radiation.
Additional Rad23 Products
Product Documents for Human/Mouse/Rat/Yeast Rad23 Antibody
Product Specific Notices for Human/Mouse/Rat/Yeast Rad23 Antibody
For research use only
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