Human/Mouse Renin R Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5716

R&D Systems, part of Bio-Techne
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AF5716
AF5716-SP
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Renin R Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Renin R
Asn17-Glu302
Accession # O75787

Specificity

Detects human and mouse Renin R in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Renin R Antibody

Detection of Human and Mouse Renin R antibody by Western Blot.

Detection of Human and Mouse Renin R by Western Blot.

Western blot shows lysates of human and mouse heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Renin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5716) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Renin R at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Renin R Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human and mouse heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Renin R

Renin R (Renin Receptor; also ATPase H+-transporting lysosomal accessory/interacting protein 2, M8.9 and ELDF10) is a 39‑45 kDa protein that belongs to no known family. It is expressed on the surface of macrophages, vascular smooth muscle cells, renal mesangial cells and T cells. Renin R has at least two functions. First, it binds both renin and prorenin, promoting the conversion of angiotensinogen to angiotensin I. Second, its ligation induces PAI‑1 synthesis. Mature human Renin R is a type I transmembrane protein 334 amino acids (aa) in length. It contains a 286 aa extracellular region (aa 17‑302) and a 27 aa cytoplasmic tail. It is believed to form homodimers. Potential isoforms exist. One termed M8.9 shows a deletion of aa 1‑252 and constitutes a component of the vacuolar proton ATPase. Two others show an alternate start site at Met77, and a deletion of aa 101‑132. Over aa 17‑302, the human Renin R shares 95% aa identity with the mouse Renin R.

Long Name

Renin Receptor

Alternate Names

ATP6AP2, ATP6IP2, ATP6M8-9, CAPER, ELDF10, HT028, M8-9, MRXE, MSTP009, N14F, ReninR, XMRE

Entrez Gene IDs

10159 (Human); 70495 (Mouse); 302526 (Rat)

Gene Symbol

ATP6AP2

UniProt

O75787

Additional Renin R Products

Product Documents for Human/Mouse Renin R Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Renin R Antibody

For research use only

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