Human/Mouse RND3 (RD1 Peptide) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6618

R&D Systems, part of Bio-Techne
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MAB6618
MAB6618-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 687202

View all RND3 Antibodies »

Product Specifications

Immunogen

RND3 (RD1 peptide)
Cys197-Arg207
Accession # P61587

Specificity

Detects human RND3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse RND3 (RD1 Peptide) Antibody

Detection of Human RND3 antibody by Western Blot.

Detection of Human RND3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human RND3 (RD1 Peptide) Monoclonal Antibody (Catalog # MAB6618) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RND3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse RND3 (RD1 Peptide) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, MCF‑7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 1 review rated 4 using MAB6618 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RND3

RND3, also known as RhoE, is an approximately 27 kDa member of the Rho family, small GTPase superfamily of molecules.  It is 244 amino acids (aa) in length, and the mature chain extends from aa 1-241.  RND3 is ubiquitously expressed and is involved in keratinocyte differentiation and stratification.  RND3 overexpression results in cell size enlargement and an increase in the number of stratified cells.  RND3 depletion results in hyperproliferation and delays initiation of keratinocyte differentiation.  Human RND3 shares 100% aa sequence identity with mouse RND3.

Long Name

Rho family GTPase 3

Alternate Names

ARHE, MemB, Rho8

Entrez Gene IDs

390 (Human)

Gene Symbol

RND3

UniProt

P61587

Additional RND3 Products

Product Documents for Human/Mouse RND3 (RD1 Peptide) Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse RND3 (RD1 Peptide) Antibody

For research use only

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