Human/Mouse ROR gamma/RORC/NR1F3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6109
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
CyTof, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 600214
Product Specifications
Immunogen
E. coli-derived recombinant human ROR gamma/RORC/NR1F3
Met1-Gln100
Accession # P51449-2
Met1-Gln100
Accession # P51449-2
Specificity
Detects human and mouse ROR gamma/RORC/NR1F3 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse ROR gamma/RORC/NR1F3 Antibody
Detection of Human and Mouse ROR gamma/NR1F3 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, EL-4 mouse lymphoblast cell line, and mouse thymus tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse ROR gamma/NR1F3 Monoclonal Antibody (Catalog # MAB6109) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROR gamma/NR1F3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse ROR gamma/RORC/NR1F3 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, EL‑4 mouse lymphoblast cell line, and mouse thymus tissue
Sample: Jurkat human acute T cell leukemia cell line, EL‑4 mouse lymphoblast cell line, and mouse thymus tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB6109 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ROR gamma/RORC/NR1F3
ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑100 human RORC shares 96% identity with mouse ROR gamma.
Long Name
Retinoic Acid-Related Orphan Receptor gamma
Alternate Names
NR1F3, RORC, RZRG
Entrez Gene IDs
6097 (Human)
Gene Symbol
RORC
UniProt
Additional ROR gamma/RORC/NR1F3 Products
Product Documents for Human/Mouse ROR gamma/RORC/NR1F3 Antibody
Product Specific Notices for Human/Mouse ROR gamma/RORC/NR1F3 Antibody
For research use only
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