Human/Mouse RPTOR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5957
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 514208
Product Specifications
Immunogen
E. coli-derived recombinant human RPTOR
Lys77-Gln230
Accession # Q8N122
Lys77-Gln230
Accession # Q8N122
Specificity
Detects human and mouse RPTOR in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse RPTOR Antibody
Detection of Human and Mouse RPTOR by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Human/Mouse RPTOR Monoclonal Antibody (Catalog # MAB5957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RPTOR at approximately 150 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of RPTOR in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RPTOR Monoclonal Antibody (Catalog # MAB5957, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of RPTOR in C2C12 Mouse Cell Line by Flow Cytometry.
C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse RPTOR Monoclonal Antibody (Catalog # MAB5957, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse RPTOR Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Neuro‑2A mouse neuroblastoma cell line
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Neuro‑2A mouse neuroblastoma cell line
Reviewed Applications
Read 1 review rated 3 using MAB5957 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RPTOR
RPTOR (Raptor) is a 150 kDa component of the cytosolic mammalian target of Rapamycin complex 1 (mTORC1) which also contains mTOR and GBL proteins. mTORC1 plays a dominant role in cell cycle regulation in response to metabolic conditions. The interaction of RPTOR with the kinase mTOR is stabilized under conditions of nutrient deprivation and energy stress, leading to inhibition of mTOR and cell cycle arrest. RPTOR contains multiple Ser and Thr residues whose phosphorylation regulates the activation status of mTOR. RPTOR is critical for the response of skeletal muscle and adipose tissue to insulin. It contains three RNC blocks (aa 48-511), three HEAT repeats (aa 550-667), and seven C-terminal WD40 domains (aa 1020-1335). Within aa 77-230, human RPTOR shares 100% aa sequence identity with mouse and rat RPTOR.
Long Name
Regulatory Associated Protein of mTOR
Alternate Names
KIAA1303, Raptor
Gene Symbol
RPTOR
UniProt
Additional RPTOR Products
Product Documents for Human/Mouse RPTOR Antibody
Product Specific Notices for Human/Mouse RPTOR Antibody
For research use only
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