Human/Mouse SEZ6L2/BSRP-A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4916
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L2/BSRP-A
Leu28-Ser837
Accession # Q4V9Z5
Leu28-Ser837
Accession # Q4V9Z5
Specificity
Detects human and mouse SEZ6L2/BSRP-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) BSRP-B and rmBSRP-C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse SEZ6L2/BSRP-A Antibody
SEZ6L2/BSRP-A in Mouse Embryo.
SEZ6L2/BSRP-A was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse SEZ6L2/BSRP-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4916) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain (medulla oblongata), spinal cord, trigeminal ganglia, and retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse SEZ6L2/BSRP-A Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse SEZ6L2/BSRP-A
Sample: Recombinant Mouse SEZ6L2/BSRP-A
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SEZ6L2/BSRP-A
SEZ6L2 (Brain-specific receptor-like protein A; also BSRP-A) is a 170 kDa member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons and likely regulates PKC alpha activation. Mature mouse SEZ6L2 is an 890 amino acid (aa) type I transmembrane protein. It contains an 824 aa extracellular domain (ECD) (aa 21-844) and a 43 aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for SEZ6L2. One shows a premature truncation after Arg403, a second shows a deletion of aa 71-130, and a third likely represents the full-length form that contains a 13 aa insertion after Val830. Over aa 28-837, mouse SEZ6L2 is 96% and 99% aa identical to human and rat SEZ6L2, respectively.
Long Name
Seizure Related 6 Homolog-Like 2/Brain-specific Receptor-like Protein-A
Alternate Names
BSRP-A, BSRPA, PSK-1
Gene Symbol
SEZ6L2
UniProt
Additional SEZ6L2/BSRP-A Products
Product Documents for Human/Mouse SEZ6L2/BSRP-A Antibody
Product Specific Notices for Human/Mouse SEZ6L2/BSRP-A Antibody
For research use only
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