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Human/Mouse sFRP-3 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM192

R&D Systems, part of Bio-Techne
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BAM192

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 133912

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human sFRP-3
Ala33-Asn325
Accession # AAB51298

Specificity

Detects human sFRP-3 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant mouse sFRP-3 is observed and no cross-reactivity with recombinant human sFRP-1, -2, or -4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human/Mouse sFRP-3 Biotinylated Antibody

Human/Mouse sFRP-3 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Mouse sFRP-3 Antibody (Catalog # MAB1921)
  • Standard: Recombinant Human sFRP-3 Protein (Catalog # 192-SF)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-3

Secreted Frizzled-related protein (sFRP-3), also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt signaling.

Long Name

Secreted Frizzled Related Protein 3

Alternate Names

Fritz, FRZB, Frzb1, sFRP3

Entrez Gene IDs

2487 (Human); 20378 (Mouse)

Gene Symbol

FRZB

UniProt

Additional sFRP-3 Products

Product Documents for Human/Mouse sFRP-3 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse sFRP-3 Biotinylated Antibody

For research use only

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