Human/Mouse SHIP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF10117
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse SHIP
Glu874-Thr941
Accession # Q9ES52
Glu874-Thr941
Accession # Q9ES52
Specificity
Detects mouse SHIP in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse SHIP Antibody
Detection of Human and Mouse SHIP by Western Blot.
Western blot shows lysates of BaF3 mouse pro-B cell line and RL human non-Hodgkin's lymphoma B cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SHIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SHIP at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SHIP by Immunoprecipitation.
PMA-treated U-937 lysates were prepared and immunoprecipitation was performed using 2.0 µg of Goat Anti-Human/Mouse SHIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10117) pre-coupled to Dynabeads protein G. Immunoprecipitated SHIP was detected with Mouse Anti-SHIP Antibody.The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).Applications for Human/Mouse SHIP Antibody
Application
Recommended Usage
Immunoprecipitation
2 µg/1 mg cell lysate
Sample: Cell lysate of U-937 cell line
Sample: Cell lysate of U-937 cell line
Western Blot
1 µg/mL
Sample: BaF3 mouse pro-B cell line and RL human non-Hodgkin's lymphoma B cell line
Sample: BaF3 mouse pro-B cell line and RL human non-Hodgkin's lymphoma B cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SHIP
SH2-containing inositol phosphatase (SHIP), also known as INPP5D, is a negative regulator of signal transduction in hematopoietic cells. Targeted disruption of SHIP in mice leads to a myeloproliferative disorder. Several laboratories have demonstrated the presence of multiple forms of SHIP, including 145 kDa, 135 kDa, and
C‑terminal truncated forms at 125 kDa and 110 kDa in some cell types.
Long Name
SH2-containing Inositol Phosphatase
Alternate Names
INPP5D
Gene Symbol
INPP5D
UniProt
Additional SHIP Products
Product Documents for Human/Mouse SHIP Antibody
Product Specific Notices for Human/Mouse SHIP Antibody
For research use only
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