Human/Mouse SIN3A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6115
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SIN3A
Tyr1187-Pro1273
Accession # Q96ST3
Tyr1187-Pro1273
Accession # Q96ST3
Specificity
Detects human and mouse SIN3A in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse SIN3A Antibody
Detection of Human SIN3A by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SIN3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6115) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SIN3A at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse SIN3A Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Sample: 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIN3A
SIN3A (Switch insensitive-3 A) is a nuclear, 140‑150 kDa member of the SIN3 family of co-repressors. SIN3A plays a key role in gene repression. Normally, genes are not expressed when chromatin is "closed", or condensed, precluding transcriptional activators from assembling on gene promoters. This state is achieved through histone deacetylation and DNA methylation, and SIN3A serves as a scaffold for multiple proteins, including deacetylases (HDAC1 and 2). Human SIN3A is 1273 amino acids (aa) in length. It contains three PAH (paired amphipathic helix) repeats (aa 141‑187, 322‑381 and 477‑523) and multiple phosphoserines and acetylated lysines. There are three potential splice variants. The first shows a 33 aa substtution for aa 124‑1273, while the second and third show eight aa substitutions for aa 337‑352, coupled to either a deletion of aa 1009‑1066, or a two aa substitution for aa 1008‑1065. Over aa 1187‑1273, human and mouse SIN3A show 100% identity in aa sequence.
Long Name
SIN3 Homolog A
Alternate Names
DKFZP434K2235, FLJ90319, Histone deacetylase complex subunit Sin3a, KIAA0700, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator (yeast), SIN3 homolog A, transcriptional regulator (yeast), Transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A
Gene Symbol
SIN3A
UniProt
Additional SIN3A Products
Product Documents for Human/Mouse SIN3A Antibody
Product Specific Notices for Human/Mouse SIN3A Antibody
For research use only
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