Human/Mouse SMOC-2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF5140
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SMOC-2
Gln22-Gly446
Accession # Q9H3U7
Gln22-Gly446
Accession # Q9H3U7
Specificity
Detects human and mouse SMOC-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human SMOC-1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human/Mouse SMOC-2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample:
Sample:
Recombinant Human SMOC‑2 (Catalog # 5140-SM)
Recombinant Mouse SMOC‑2 (Catalog # 6075-SM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMOC-2
SMOC-2 (secreted modular calcium-binding protein 2; also SMAP2) is a 55 kDa glycoprotein member of the SPARC family of matricellular proteins. It is widely expressed, perhaps by endothelium and apparently participates in both growth factor signaling and ECM organization. Mature human SMOC-2 is 425 amino acids (aa) in length. It contains one Kazal-like domain (aa 40‑84), two thyroglobulin type-1 segments (aa 87‑153 and 213‑281) and two EF-hand sequences (aa 347‑382 and 384‑419). There are three splice variants. One shows a 13 aa substitution for aa 442‑446, another shows an 11 aa insertion after Thr170 and a third represents a combination of the above two variations. Over aa 22‑446, human SMOC-2 shares 95% aa identity with mouse SMOC-2.
Long Name
Secreted Modular Calcium-binding Protein 2
Alternate Names
MST117, MSTP117, MSTP140, SMAP2, SMOC2
Gene Symbol
SMOC2
UniProt
Additional SMOC-2 Products
Product Documents for Human/Mouse SMOC-2 Biotinylated Antibody
Product Specific Notices for Human/Mouse SMOC-2 Biotinylated Antibody
For research use only
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