Human/Mouse SPRY2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6157
Key Product Details
Species Reactivity
Human, Mouse
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SPRY2
Met1-Ala175
Accession # O43597
Met1-Ala175
Accession # O43597
Specificity
Detects human and mouse SPRY2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse SPRY2 Antibody
Detection of Human and Mouse SPRY2 by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, PC-3 human prostate cancer cell line, and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SPRY2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SPRY2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse SPRY2 by Simple WesternTM.
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and SH-SY5Y human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPRY2 at approximately 45 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse SPRY2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6157) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse SPRY2 Antibody
Application
Recommended Usage
Simple Western
50 µg/mL
Sample: C2C12 mouse myoblast cell line and SH‑SY5Y human neuroblastoma cell line
Sample: C2C12 mouse myoblast cell line and SH‑SY5Y human neuroblastoma cell line
Western Blot
1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, PC‑3 human prostate cancer cell line, and C2C12 mouse myoblast cell line
Sample: SH‑SY5Y human neuroblastoma cell line, PC‑3 human prostate cancer cell line, and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SPRY2
SPRY2 (sprouty homolog 2) is a 35 kDa member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKC delta and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 amino acids (aa) in length and contains one CBL-TKB binding site (aa 53‑59) that is phosphorylated at Tyr55, a Ser-rich region (aa 125‑131), a Cys-rich domain (aa 178‑301) and a PxxPxR motif that mediates ERK inhibitory activity. Over aa 1‑175, human SPRY2 shares 94% aa identity with mouse SPRY2.
Long Name
Sprouty Homolog 2
Alternate Names
sprouty 2
Gene Symbol
SPRY2
UniProt
Additional SPRY2 Products
Product Documents for Human/Mouse SPRY2 Antibody
Product Specific Notices for Human/Mouse SPRY2 Antibody
For research use only
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