Human/Mouse UCP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4739
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human UCP2
Met1-Phe309
Accession # P55851
Met1-Phe309
Accession # P55851
Specificity
Detects human and mouse UCP2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) UCP1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse UCP2 Antibody
Detection of Human/Mouse UCP2 by Western Blot.
Western blot shows lysates of mouse brown adipose tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human UCP1, UCP2, UCP3, and UCP4 (5 ng/lane) were included. A specific band for UCP2 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Mouse UCP2 by Simple WesternTM.
Simple Western lane view shows lysates of mouse brown adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for UCP2 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse UCP2 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Mouse brown adipose tissue
Sample: Mouse brown adipose tissue
Western Blot
1 µg/mL
Sample: Mouse brown adipose tissue
Sample: Mouse brown adipose tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UCP2
Long Name
Uncoupling Protein 2
Alternate Names
SLC25A8, UCPH
Gene Symbol
UCP2
UniProt
Additional UCP2 Products
Product Documents for Human/Mouse UCP2 Antibody
Product Specific Notices for Human/Mouse UCP2 Antibody
For research use only
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