Human/Mouse WASF1/WAVE1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5514
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human WASF1/WAVE1
Met1-Tyr250
Accession # Q92558
Met1-Tyr250
Accession # Q92558
Specificity
Detects endogenous human and mouse WASF1/WAVE1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse WASF1/WAVE1 Antibody
Detection of Human/Mouse WASF1/WAVE1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A172 human glioblastoma cell line, and 293T human embryonic kidney cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse WASF1/WAVE1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5514) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WASF1/WAVE1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse WASF1/WAVE1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, A172 human glioblastoma cell line, and 293T human embryonic kidney cell line, and mouse brain tissue
Sample: K562 human chronic myelogenous leukemia cell line, A172 human glioblastoma cell line, and 293T human embryonic kidney cell line, and mouse brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WASF1/WAVE1
WASF1/WAVE1 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 1) is an 80‑85 kDa member of the SCAR/WAVE family of proteins. It is expressed in diverse cell types such as neurons, fibroblasts, macrophages, platelets, oligodendroglia and breast epithelium. WASF1/WAVE1 is normally inactive, being phosphorylated at Ser310 and complexed with HSPC300, PIR121 and Nap125. Upon activation, it is dephosphorylated and decoupled, heterodimerizes with WASP, and initiates actin polymerization. Human WASF1/WAVE1 is 559 amino acids (aa) in length. It contains five sequential poly-Pro regions (aa 278‑435) and one VPH domain (aa 497‑514) that binds to actin and Arp2/3. There is one isoform that shows a 23 aa N-terminal extension. In mouse, a 60 kDa proteolytic cleavage form is reported. Over aa 1‑250, human WASF1/WAVE1 shares 99% aa identity with mouse WASF1/WAVE1.
Long Name
Wiskott-Aldrich Syndrome Protein Family Member 1
Alternate Names
SCAR1, WASP1, WAVE1
Gene Symbol
WASF1
UniProt
Additional WASF1/WAVE1 Products
Product Documents for Human/Mouse WASF1/WAVE1 Antibody
Product Specific Notices for Human/Mouse WASF1/WAVE1 Antibody
For research use only
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