Human/Mouse Wnt-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB475
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 55010
Product Specifications
Immunogen
E. coli-derived recombinant mouse Wnt-4
aa 37-76 and 222-295
Accession # P22724
aa 37-76 and 222-295
Accession # P22724
Specificity
Detects mouse Wnt-4 in direct ELISAs and Western blots. In sandwich immunoassays, this antibody is specific for human Wnt-4 as a capture antibody when paired with Sheep
Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF6076)
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse Wnt-4 Antibody
Wnt‑4 in Mouse Embryo.
Wnt-4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rat Anti-Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB475) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Human Wnt-4 ELISA Standard Curve.
Recombinant Human Wnt-4 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB475) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).Applications for Human/Mouse Wnt-4 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody for Human Wnt-4 when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
Western Blot
1 µg/mL
Sample: Recombinant Mouse Wnt-4 (Catalog # 475-WN)
Sample: Recombinant Mouse Wnt-4 (Catalog # 475-WN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-4
Long Name
Wingless-type MMTV Integration Site Family, Member 4
Alternate Names
Wnt4
Gene Symbol
WNT4
UniProt
Additional Wnt-4 Products
Product Documents for Human/Mouse Wnt-4 Antibody
Product Specific Notices for Human/Mouse Wnt-4 Antibody
For research use only
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